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Protocol Information

Native Plant Nursery
USDI NPS - Glacier National Park
West Glacier, Montana 59936
(406) 888-7835


Family Scientific Name: Caprifoliaceae
Family Common Name: Honeysuckle family
Scientific Name: Lonicera involucrata (Rich.) Banks. ex Spreng. involucrata Banks. ex Spreng.
Common Synonym: Xylosteum involucratum (Banks ex Spreng.) Richards.
Common Name: Black twinberry
Species Code: LONINV
Ecotype: Lodgepole Forest, Many Glacier, 1500m elev.
General Distribution: L. involucrata occurs from Alaska south to California and Mexico, east to Montana to New Mexico, and irregularly Michigan and Quebec. It is found from valley bottoms to subalpine zone in moist to wet soils.
Propagation Goal: Plants
Propagation Method: Seed
Product Type: Container (plug)
Stock Type: 172 ml conetainer
Time To Grow: 9 Months
Target Specifications: Height: 17 cm
Caliper: 7 mm
Root System: firm plug in conetainer.
Propagule Collection: Fruit is collected in August. Hand collect when the fruit turns black. Seeds are tan at maturity. Keep fruit in plastic bags under refrigeration until it is cleaned.
Propagule Processing: Seeds are extracted from the fruit by maceration using the Dyb-Vig cleaner and is washed and screened.
Seed Storage:up to 15 years at 1 to 3C in sealed containers.
Seed dormancy is classified as morpho-physiological dormancy.
Seeds/Kg: 718,000/kg
% Purity: 100%
% Germination: 40% without gibberellic acid treatment, 97% with gibberellic acid treatment.
Pre-Planting Treatments: Seeds were pretreated with 1000 ppm gibberellic acid for 24 hours. Following treatment, seeds were placed into a 48 hour running water rinse. Next, seeds were placed into cold, moist stratification for 90 days. Seeds are placed in fine mesh bags and buried in moist peat moss in ventilated containers under refrigeration at 3 C.
Growing Area Preparation/
Annual Practices for Perennial Crops:

Greeenhouse.
Sowing Method: Planting Germinants. Seeds are covered with medium.
Growing medium used is 6:1:1 milled sphagnum peat, perlite, and vermiculite with Osmocote controlled release fertilizer (13N:13P2O5:13K2O; 8 to 9 month release rate at 21C) and Micromax fertilizer (12%S, 0.1%B, 0.5%Cu, 12%Fe, 2.5%Mn, 0.05%Mo, 1%Zn) at the rate of 1 gram of Osmocote and 0.20 gram of Micromax per 172 ml conetainer.
Establishment Phase: Germination was uniform and completed in 2 weeks when greenhouse temperatures are set at 22/15C alternating day/night) temperature cycle.
Length of Establishment Phase: 4 weeks
Active Growth Phase: Plants were fertilized weekly with 20-20-20 liquid NPK at 100 ppm. This species requires frequent irrigation.
Length of Active Growth Phase: 16 weeks
Hardening Phase: Plants were fertilized with 10-20-20 liquid NPK at 200 ppm during September and October and given one final irrigation before overwintering.
Length of Hardening Phase: 4 weeks
Harvesting, Storage and Shipping: Total Time to Harvest: 9 months
Harvest Date: September
Storage Conditions: Overwinter in outdoor nursery under insulating foam cover and snow.
Length of Storage: 5 months
Outplanting performance on typical sites: Outplanting Site: Logan Pass, Glacier National Park, MT.
Outplanting Date: After snowmelt at high elevations.
Outplanting Survival at 3 years: 100%
Other Comments: This species develops an extensive root system rapidly and will require yearly uppotting if it is to be held in the nursery for more than 1 year. 3 L (1 gallon) container stock can be produced in 1.5 years.
Both seed and cutting propagation give good rates of success.
There are 2 botanical varieties; var. involucrata, and var. ledebourii.
References: Flora of the Pacific Northwest, Hitchcock and Cronquist, University of Washington Press, 7th printing, 1990.

Seeds of the Woody Plants in North America, Young and Young, Dioscorides Press, 1992.

The Reference Manual of Woody Plant Propagation: From Seed to Tissue Culture, Dirr and Heuser, Varsity Press, 1987.

1999 Revegetation Monitoring Report, Glacier National Park, Asebrook, J. and Brenneman, B., unpublished.

Citation:
Luna, Tara; , Tara Luna.; Wick, Dale. 2008. Propagation protocol for production of container Lonicera involucrata (Rich.) Banks. ex Spreng. involucrata Banks. ex Spreng. plants (172 ml conetainer); USDI NPS - Glacier National Park, West Glacier, Montana. In: Native Plant Network. URL: http://www.nativeplantnetwork.org (accessed 1 September 2014). Moscow (ID): University of Idaho, College of Natural Resources, Forest Research Nursery.
 

Protocol Information

Betty Young
Nurseries Sr Manager
Bldg 201, Fort Mason
San Francisco, California 94123
415-331-6917
415-331-7521
byoung@ggnpa.org


Family Scientific Name: Caprifoliaceae
Family Common Name: Honeysuckle Family
Scientific Name: Lonicera involucrata (Richards) ledebourii (Esch.) Zebel
Common Synonym: Lonicera ledebourii Eschsch.
Common Name: Twinberry Honeysuckle
Species Code: LONINV
Ecotype: Marin County, California
General Distribution: L. involucrata var.ledebourii is the coastal variety that is found from Central California north to British Columbia in wooded habitats.
Propagation Goal: Plants
Propagation Method: Seed
Product Type: Container (plug)
Stock Type: Deepot 40
Target Specifications: Height: N/A
Caliper: N/A
Root System: Firm plug in container.
Propagule Collection: Seeds are collected between April 1st and July 1st.
Mature fruits are bluish black.
Seed is hard at maturity.
Propagule Processing: Seed Cleaning:Mash berries in a bucket, wash until seeds are free and pour through a sieve. Repeat and dry seeds.
Storage Conditions: Seeds are kept dry and stored in a refrigerator.
Pre-Planting Treatments: Soak seeds in fresh water for 12 hours. Cold stratify in peat or perlite for 3 to 6 months.
Growing Area Preparation/
Annual Practices for Perennial Crops:

Fully Controlled Greenhouse.
Sowing Method: Transplanting Germinants.
6 grams of seeds are sown per flat containing Sunshine Mix #4 Aggregate Plus (peat moss, perlite, major and minor nutrients, gypsum, and dolomitic lime).
Seeds are covered with media.
Flats are watered in with an automatic mist and irrigation system.
Seeds are sown on June 15th.
% Germination:50%
Establishment Phase: Seeds germinate 14 days after sowing.
Seedlings are transplanted 14 days after germination to individual containers 2"x8" tubes (Deepot 40) containing standard potting mix of peat moss, fir bark, perlite, and sand.
Transplant Survival averages 90%.
Length of Establishment Phase: 28 days
Active Growth Phase: Fertilize with Nutricote NPK (13-13-13) 3 months after transplFertilize with Nutricote NPK (13-13-13) 1 month after transplanting.
Cut back 2 months after transplanting, leaving at least 4 nodes. Repeat as needed.
Twinberry is a rapid growing species.
References: A California Flora and Supplement, Munz, P., University of California Press, Berkeley and London, 1973.

Citation:
Young, Betty 2001. Propagation protocol for production of container Lonicera involucrata (Richards) ledebourii (Esch.) Zebel plants (Deepot 40); , San Francisco, California. In: Native Plant Network. URL: http://www.nativeplantnetwork.org (accessed 1 September 2014). Moscow (ID): University of Idaho, College of Natural Resources, Forest Research Nursery.
 

Protocol Information

Betty Young
Nurseries Sr Manager
Bldg 201, Fort Mason
San Francisco, California 94123
415-331-6917
415-331-7521
byoung@ggnpa.org


Family Scientific Name: Caprifoliaceae
Family Common Name: Honeysuckle Family
Scientific Name: Lonicera involucrata Banks ex Spreng.
Common Name: Black Twinberry Honeysuckle
Species Code: LONINV
Ecotype: Marin County, California
General Distribution: L. involucrata is found from Alaska south to California and Mexico, east to Montana to New Mexico, and irregularly to Michigan and Quebec. It is found from sea level to valley bottoms to subalpine zone in moist to wet soils.
Propagation Goal: Plants
Propagation Method: Vegetative
Product Type: Container (plug)
Stock Type: Deepot 40
Target Specifications: Height: N/A
Caliper: N/A
Root System: Firm plug in container.
Propagule Collection: Hardwood cuttings are collected between November 1st and January 15th.
Cutting diameter is 0.5 inches.
Cutting length is 10 inches with at least 5 nodes.
Cuttings should be collected before any sign of bud break.
Propagule Processing: Cuttings are kept moist and cool prior to treatment.
Pre-Planting Treatments: Cuttings are dipped in a mild bleach solution for 30 seconds.
Cuttings are treated with Hormex (3000 ppm IBA) rooting powder and struck in flats containing 3:1 Perlite/Vermiculite.
50 Cuttings are struck 2.5 inch deep per flat.
% Rooting: 50%
Growing Area Preparation/
Annual Practices for Perennial Crops:

Fully Controlled Greenhouse.
Flats are kept in the greenhouse and watered with an automatic mist system until roots are fully developed.
Establishment Phase: Planting Method: Transplanting Cuttings.
Time to Transplant: 50 days.
Cuttings are transplanted to individual containers 2"x10" tubes (Deepot 40) containing standard potting mix of peat moss, fir bark, perlite, and sand. Cuttings are placed in the shadehouse.
Transplant Survival averages 75%.
References: A California Flora and Supplement, Munz, P., University of California Press, Berkeley and London, 1973.

Citation:
Young, Betty 2001. Propagation protocol for vegetative production of container Lonicera involucrata Banks ex Spreng. plants (Deepot 40); , San Francisco, California. In: Native Plant Network. URL: http://www.nativeplantnetwork.org (accessed 1 September 2014). Moscow (ID): University of Idaho, College of Natural Resources, Forest Research Nursery.
 

Protocol Information

Lindsay Springer
Lead Biological Science Technician - Greenhouse Manager
USDI NPS - Rocky Mountain National Park
1000 Highway 36
Estes Park, Colorado 80517-8397
(970) 586-1252
(970) 589-1392
Lindsay_Springer@nps.gov


Family Scientific Name: Caprifoliaceae
Scientific Name: Lonicera involucrata
Common Name: Twinberry
Ecotype: Colorado, GMV (98-031c, 98-089c)
Propagation Goal: Plants
Propagation Method: Vegetative
Product Type: Container (plug)
Propagule Collection: Collected at GMV (98-031c) on 6/19/98; GMV (98-089c) 7/28/98.
Pre-Planting Treatments: Treatment: GMV (98-031c) treated with rootinghor; GMV (98-089c) treated 15:1 rh.
Growing Area Preparation/
Annual Practices for Perennial Crops:

Sowing/planting technique: GMV (98-031c) media per/ver and located with mister; GMV (98-089c) media ver/per/germ with 25% root, transplanted 8/28/98 and located with mister.

Citation:
Butler, Jennifer; Frieswyk, Christin. 2001. Propagation protocol for vegetative production of container Lonicera involucrata plants; USDI NPS - Rocky Mountain National Park, Estes Park, Colorado. In: Native Plant Network. URL: http://www.nativeplantnetwork.org (accessed 1 September 2014). Moscow (ID): University of Idaho, College of Natural Resources, Forest Research Nursery.