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Protocol Information

Native Plant Nursery
USDI NPS - Glacier National Park
West Glacier, Montana 59936
(406) 888-7835

Family Scientific Name: Pinaceae
Family Common Name: Pine family
Scientific Name: Pinus contorta Dougl.ex Loud. latifolia Engelm. ex S. Wats.
Common Name: Lodgepole pine
Species Code: PINCON
Ecotype: Lodgepole forest, Many Glacier, Glacier National Park, Glacier Co., MT, 1585m elevation.
General Distribution: P. contorta var. latifolia is found in montane dry to moist areas, often forming pure stands;from southern Alaska and Yukon, southward to northern Baja California, east to Alberta and extreme southwestern Saskatchewan, South Dakota, and through the Rocky Mountains to Colorado. It is also common throughout the northeastern Oregon, Idaho, northern Utah, and extreme western Nevada.
Propagation Goal: Plants
Propagation Method: Seed
Product Type: Container (plug)
Stock Type: 172 ml conetainers
Time To Grow: 9 Months
Target Specifications: Stock Type: Container seedling.
Height: 16 cm.
Caliper: n/a.
Root System: firm plug in conetainer.
Propagule Collection: Serotinous and non serotinous cones are collected in early September. Mature seeds are firm and brown in color.
Cones are collected by cutting branches with pruning poles. Cones are removed from branches with pruning shears and stored in burlap bags in a well ventilated drying shed.
Propagule Processing: Serotinous cones are heated in kilns or placed in boiling water and then air dried to facilitate opening and seed extraction. A tumbler and dewinger are used to clean large quantities.
Seed longevity: is up to 25 years at 1 to 3C in sealed containers.
Seed dormancy is classified as physiological dormancy.
Seeds/Kg: 208,000 /kg
% Purity: 100%
% Germination: 80 to 94%
Pre-Planting Treatments: Seeds are treated with 8:1(v:v)water/bleach bath for 10 minutes, followed by a 48 hour running water rinse soak and a 45 day cold, moist stratification.
Seeds are placed in fine mesh bags in moist peat moss and buried in ventilated containers at 3C.
Nonstratified P. contorta seeds are reported to germinate to higher percentages in light than in darkness. Stratified seeds germinate to higher percentages in darkness.
Prechilling may promote overall germination energy and uniformity.
Growing Area Preparation/
Annual Practices for Perennial Crops:

Greenhouse and outdoor nursery growing facility.
Sowing Method: Direct Seeding. Stratified seeds are lightly covered with medium.
Growing medium used is 6:1:1 milled sphagnum peat, perlite, and vermiculite with Osmocote controlled release fertilizer (13N:13P2O5:13K2O; 8 to 9 month release rate at 21C) and Micromax fertilizer (12%S, 0.1%B, 0.5%Cu, 12%Fe, 2.5%Mn, 0.05%Mo, 1%Zn) at the rate of 1 gram of Osmocote and 0.20 gram of Micromax per 172 ml conetainer.
Greenhouse temperatures are maintained at 21 to 25C during the day and 16 to 18C at night. Seedlings are hand watered and remain in greenhouse until mid May. Seedlings are then moved to outdoor nursery for the remainder of the growing season.
Establishment Phase: Medium is kept slightly moist during germination. Seeds must not be sown or covered too deeply. Initial germination is typically complete in 20 days. Germinants shed the seed coats 14 days after emergence. Seedlings are thinned at the birdcage stage.
Accelerated growth is usually reached by week 8.
Length of Establishment Phase: 8 weeks
Active Growth Phase: Plants were fertilized with 25-10-10 liquid NPK at 200 ppm during the growing season. Seedlings can be inoculated with mycorrhizal fungi at this stage. Plants were fully root tight 23 weeks after germination and averaged 16 cm in height.
Length of Active Growth Phase: 16 weeks
Hardening Phase: Tree seedlings are fertilized with 10-20-20 liquid NPK at 200 ppm in August and September. Irrigation is gradually reduced in September and October. Seedlings are flushed with clear water once before winterization.
Length of Hardening Phase: 4 weeks
Harvesting, Storage and Shipping: Total Time To Harvest: 9 months
Harvest Date: September and October
Storage Conditions: Overwinter in outdoor nursery under insulating foam cover and snow.
Length of Storage: 5 months
Outplanting performance on typical sites: Outplanting Site: Two Medicine, Glacier National Park, MT.
Outplanting Date: Spring or Fall.
Other Comments: One gallon container stock can be produced in 2 years with an average height of 37 cm and 1 cm caliper.
There are 2 botanical varieties; var. contorta, which is the coastal variety, and var. latifolia.
References: Flora of the Pacific Northwest, Hitchcock and Cronquist, 7th edition, University of Washington Press, 1973.

Seeds: Ecology, Biogeography, and Evolution of Dormancy and Germination, Baskin and Baskin, Academic Press, 1998.

Seeds of Woody Plants in North America, Young and Young, Dioscorides Press, 1992.

Glacier National Park Native Plant Nursery Propagation Records, unpublished.

Luna, Tara; Evans, Jeff.; Hosokawa, Joy.; Wick, Dale. 2008. Propagation protocol for production of container Pinus contorta Dougl.ex Loud. latifolia Engelm. ex S. Wats. plants (172 ml conetainers); USDI NPS - Glacier National Park, West Glacier, Montana. In: Native Plant Network. URL: http://www.nativeplantnetwork.org (accessed 4 September 2015). Moscow (ID): University of Idaho, College of Natural Resources, Forest Research Nursery.

Protocol Information

Rae Watson
Forestry Technician
USDA FS - J Herbert Stone Nursery
2606 Old Stage Rd.
Central Point, Oregon 97537

Family Scientific Name: Pinaceae
Scientific Name: Pinus contorta latifolia
Common Name: Lodgepole pine
Propagation Goal: Plants
Propagation Method: Seed
Product Type: Bareroot (field grown)
Stock Type: 1+0
Time To Grow: 10 Months
Target Specifications: Minimum height is 3in and minimum caliper is 4mm.
Propagule Collection: Most seed comes from wild collections, with the remainder coming from seed orchards managed by the Forest Service and Bureau of Land Management. All seed is kept separate by the collection area, elevation and date collected. All seed is collected or contracted for collection by the Forest Service and Bureau of Land Management or other government agencies. All seed is collected in the fall.
Propagule Processing: Seed is sent to Bend Pine Extractory in the fall for cleaning. It is dried to between 5 and 8% moisture and placed in air tight plastic bags, then stored in seed freezers set at -15C (5F) at the nursery. This seed has a long storage life under these conditions.
Pre-Planting Treatments: Seed is placed in mesh bags and soaked in cold running water for 48 hour. The seed is then laid out 3cm (1 in) thick on trays with fine screen meshed bottoms and placed in cold stratification rooms for 30 to 45 days. Rooms are equipped with foggers to keep the naked seed moist at all times (seed covered with free moisture). Temperatures are set at 1C (33F). Seed is monitored daily to detect seed mold. If mold is found, the seed is hosed down with water.
Growing Area Preparation/
Annual Practices for Perennial Crops:

The nursery soils are a sandy loam (Central Point Sandy Loam Soil Series – Coarse-loamy, mixed, mesic Pachic Haploxeroll). Nine months before sowing, in late spring, 2.5cm (1in) inch of fresh sawdust is applied and disked into the surface. During the summer, the fields are irrigated to encourage weeds to sprout. The fields are disked at regular intervals to keep them free of weeds. Soils are formed into 1.2m (4ft) wide raised beds separated by a 0.6m (2ft) tractor path. There are six beds between irrigation pipelines.
Establishment Phase: Seeds are the first to be sown at the nursery (mid-March). Seed is sown through a modified Oyjard seed drill. Seed is sown for an initial seedling density of 194 seedlings/m2 (18 seedlings/ft2). Attached to the front of the seed drill is a fertilizer bander. Depending on our soil analysis the bander places 500 kg/ha (450 lb/ac) of potassium sulfate (0-0-53) and 400 kg/ha (360 lb/ac) of ammonium phosphate (11-52-0) is placed at a depth in the soil of10cm (4in). The seed drill has been adapted by attaching 8 steel bands to the drum. The bands are 3cm (1¼ in) wide by 1cm (3/8in) deep and 15cm (6 in) apart. As it rolls in front of the seeder, the band creates a small impression for the seed to drop into. The tubes of the seed drill have been increased in size to allow large seed to pass through and drop directly into the impressions. Behind the seed tubes are small wheels that press the seed into the surface of the soil. Within a half hour of sowing, and then covered with 1cm to 1.3cm (3/8 to ½ in) of fresh (undecomposed) sawdust. The sawdust is sprayed with Agrilock at 15% solution to hold it in place in case of high winds. Then the seedbeds are sprayed with Goal (oxyfluorfen) at 2 pints per acre as a pre-emergent control for weeds.The seedbeds are irrigated when the seed appears to be drying out. This occurs only on warm days. There is no fertilization during this period.
Length of Establishment Phase: 4 weeks
Active Growth Phase: Irrigation: Soil tensiometers are placed at 15cm (6in) depths and monitored at least once per week. Soils are irrigate to 30cm (12in) when tensions are at -0.2 or higher. Light (5 minute) bursts of irrigation are given when surface soil temperatures (temperature probe placed under a ¼ inch of soil) are 33C (91F) in June; 35C (95F) in July; 38C (100F) in early August and 40C (104F) in mid August. Fertilizer: Fertilizer is applied in granular form over the seedlings. After application is complete, the fertilizer is washed off the foliage and into the soil with a half hour of irrigation water. Four applications are made: Approximately 6 weeks after emergence, 56 kg/ha (50 lbs/ac) of ammonium nitrate is applied when lateral roots have developed from new germinants. 8 weeks after emergence – 84 kg/ha (75 lbs/ac of ammonium nitrate. 10 weeks after emergence – 181 kg/ha (162 lbs/ac) ammonium sulfate, 12 weeks after emergence – 140 kg/ha (125 lbs/ac) of ammonium nitrate and 14 weeks after emergence – 168 kg/ha(150 lbs/ac) of ammonium nitrate. IPM: Handweeding of beds if necessary. If lygus insect found to damage buds, utilize mechanical insect control (Bug suck vacuum) and isecticide (Pydrin) at 10 day intervals until damage from insect no longer observed.
Length of Active Growth Phase: 4 months
Hardening Phase: By the third week in August or when the seedlings dormancy is induced. Irrigation: Only irrigate when the surface temperatures exceed 38C (100F) or pre-dawn plant moisture stress (PMS) exceeds 10 bars. In the early fall the soil profile is completely moistened and plants are kept below 5 bars pre-dawn PMS. From October through the early portion of November, the seedlings are protected from frosts through irrigation. Fertilizer: Two applications applied two weeks apart of112 kg/ha (100 lbs/ac) of ammonium sulfate. This is applied in mid-fall after bud set. IPM: Handweed beds if needed. Prunes and wrenches: Wrench at 12 inches in late August to enhance fall root activity. Vertical prune in October.
Length of Hardening Phase: 2 months
Harvesting, Storage and Shipping: Lifted the first and second week of December. Seedlings are hand-lifted after the seedlings beds have been undercut using an Lundeby lifter. Lifting conditions must be in unsaturated soils, PMS below 15 bars and temperatures above –3C (27F). Seedlings are stored at 1C (33F) and 100 percent humidity for 1 to 5 days before sorting. Sorting removes seedlings that do not meet target specifications (see above). Many clients ask for seedlings to be rootpruned between 23 and 30cm (9 and 12 inch) for planting reasons. We accomplish this with paper cutters. At clients request, we will place a rubber band around a group of seedlings, usually 25. Seedlings are placed in 3 ply bags and sown shut. The bags are placed on racks and stored in coolers at 1C (33F) for storage durations less than 2 months or in freezers at –1C (29F) for greater than 2 months.
Length of Storage: Up to 6 months
References: Schopmeyer C.S. 1974. Seeds of Woody Plants in the United States. Ag Handbook 450. USDA Forest Service. http://www.wpsm.net/

Duryea M.L., Landis T.D. 1984. Forest Nursery Manual: Production of Bareroot Seedlings. Martius Nijhoff/Dr W. Junk Publishers, the Hague Boston/Lancaster, Forest Research Lab, OSU Corvallis. 386p.

Steinfeld, David E 2001. Propagation protocol for production of field-grown Pinus contorta latifolia plants (1+0); USDA FS - J Herbert Stone Nursery, Central Point, Oregon. In: Native Plant Network. URL: http://www.nativeplantnetwork.org (accessed 4 September 2015). Moscow (ID): University of Idaho, College of Natural Resources, Forest Research Nursery.

Protocol Information

Carol and Jerry Baskin
University of Kentucky
University of Kentucky
Lexington, Kentucky 40506-0225

Family Scientific Name: Pinaceae
Family Common Name: Pine family
Scientific Name: Pinus contorta Douglas ex Loudon
Common Name: Lodgepole pine
Species Code: PINCON
General Distribution: P. contorta is found from Alaska,Yukon, and Northwest Territories east and south to Saskatchewan, South Dakota, Colorado, Wyoming, Utah, Oregon, Washington, Idaho and Montana.
Propagation Goal: Plants
Propagation Method: Seed
Product Type: Container (plug)
Propagule Processing: Seeds are non dormant.
Pre-Planting Treatments: Seeds are placed in cold moist stratification for 21 days. Germination occurs at 24 to 27 C.
Germination occurred equally well in light and dark.
References: Haasis, F. W. and Thrupp, A. C. (1931). Temperature relations of lodgepole-pine seed germination. Ecology 12, 728-744.
Table 10.35 In: Baskin, C.J. and Baskin, J.M. Seeds: Ecology, Biogeography and Evolution in Dormancy and Germination, Academic Press, 1998. Chapter 10: A Geographical Perspective on Germination Ecology: Temperate and Arctic Zones, pages 331 to 458.

Baskin, Carol C.; Baskin, Jerry M. 2002. Propagation protocol for production of container Pinus contorta Douglas ex Loudon plants; University of Kentucky, Lexington, Kentucky. In: Native Plant Network. URL: http://www.nativeplantnetwork.org (accessed 4 September 2015). Moscow (ID): University of Idaho, College of Natural Resources, Forest Research Nursery.